Bruno Lapied 1 AuthorId : Author. Hide details. Abstract : Insect nicotinic acetylcholine nACh receptors are molecular targets of insecticides such as neonicotinoids that are used to control disease-carrying insects and agricultural pests. To date, several insect nACh receptor subunits have been identified, indicating different nACh receptor subtypes and pharmacological profiles. Because of the difficulty in expressing functional insect nACh receptors in heterologous systems, new research tools are needed.
Studies on insects resistant to the insecticide imidacloprid and on laboratory-generated hybrid and chimaeric nACh receptors in vitro have provided information about the molecular basis of receptor diversity, neonicotinoid resistance and selectivity. The carbamidomethylation of cysteine was considered as a fixed modification, and the conversion of N-terminal glutamine to pyroglutamic acid and methionine oxidation were considered as variable modifications.
All identified peptides had an ion score above the Mascot peptide identity threshold, and a protein was considered to be identified if at least one such unique peptide match was apparent for the protein. The information of the sequences has been released to the database of GeneBank. The cRNAs of all subunits were generated as described previously Xenopus oocyte preparation and cRNA injection were performed as described previously Differences in responses currents , I max and EC 50 values were analyzed by one-way ANOVA with at least three repeats different batches of oocytes from different frogs.
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Insect Nicotinic Acetylcholine Receptors
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- Diversity of insect nicotinic acetylcholine receptor subunits.?
- Research Output.
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Diversity of insect nicotinic acetylcholine receptor subunits.
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